Isopentenyl diphosphate isomerase activity in Lactarius mushrooms

被引：2
|
作者：
Ohya, N
Tanaka, Y
Ogura, K
Koyama, T
机构：
[1] TOKYO UNIV AGR & TECHNOL, FAC TECHNOL, KOGANEI, TOKYO 184, JAPAN
[2] TOHOKU UNIV, INST CHEM REACT SCI, AOBA KU, SENDAI, MIYAGI 980, JAPAN
[3] TOHOKU UNIV, FAC ENGN, DEPT BIOCHEM & ENGN, AOBA KU, SENDAI, MIYAGI 98077, JAPAN
来源：
PHYTOCHEMISTRY | 1997年 / 46卷 / 06期
关键词：
Lactarius sp; mushroom; natural rubber; biosynthesis; polyisoprene; isopentenyl diphosphate; isopentenyl diphosphate isomerase;
D O I：
10.1016/S0031-9422(97)00410-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isopentenyl diphosphate (IDP) isomerase [EC 5.3.3.2] activity, which is presumed to be involved in rubber biosynthesis, was detected in a homogenate of Lactarius mushrooms by measuring the radioactive C-5, alcohols derived from [1-C-14] IDP. Fluoride ions were added to the assay mixture to inhibit phosphatase activity, which interfered with the isomerase activity by hydrolysing the IDP substrate. The level of formation of [1-C-14] DMADP in the homogenate of L. volemus was 41%. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：1115 / 1118
页数：4
相关论文
共 50 条
  • [31] Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana
    Michael Campbell
    Frederick M. Hahn
    C. Dale Poulter
    Thomas Leustek
    Plant Molecular Biology, 1998, 36 : 323 - 328
  • [32] Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata
    Yu, Xinyi
    Chen, Jiao
    Yan, Han
    Huang, Xue
    Chen, Jieru
    Ma, Zichun
    Zhou, Jiayu
    Liao, Hai
    HORTICULTURAE, 2024, 10 (08)
  • [33] Kinetic and Binding Studies of Streptococcus pneumoniae Type 2 Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase
    Janczak, Matthew Walter
    Poulter, C. Dale
    BIOCHEMISTRY, 2016, 55 (15) : 2260 - 2268
  • [34] Type-2 Isopentenyl Diphosphate Isomerase: Evidence for a Stepwise Mechanism
    Heaps, Nicole A.
    Poulter, C. Dale
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (47) : 19017 - 19019
  • [35] Isopentenyl diphosphate isomerase, a cholesterol synthesizing enzyme, is localized in Lewy bodies
    Nakamura, Keiko
    Mori, Fumiaki
    Tanji, Kunikazu
    Miki, Yasuo
    Yamada, Masahito
    Kakita, Akiyoshi
    Takahashi, Hitoshi
    Utsumi, Jun
    Sasaki, Hidenao
    Wakabayashi, Koichi
    NEUROPATHOLOGY, 2015, 35 (05) : 432 - 440
  • [36] Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana
    Campbell, M
    Hahn, FM
    Poulter, CD
    Leustek, T
    PLANT MOLECULAR BIOLOGY, 1998, 36 (02) : 323 - 328
  • [37] Biosynthesis of isoprenoids in Escherichia coli:: stereochemistry of the reaction catalyzed by isopentenyl diphosphate:dimethylallyl diphosphate isomerase
    Leyes, AE
    Baker, JA
    Hahn, FM
    Poulter, CD
    CHEMICAL COMMUNICATIONS, 1999, (08) : 717 - 718
  • [38] A guanidinium-based inhibitor of a type I isopentenyl diphosphate isomerase
    Abdelmagid, Walid M.
    Mahmoodi, Niusha
    Tanner, Martin E.
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2020, 30 (22)
  • [39] Crystal structure of the type II isopentenyl diphosphate:: Dimethylallyl diphosphate isomerase from Bacillus subtilis
    Steinbacher, S
    Kaiser, J
    Gerhardt, S
    Eisenreich, W
    Huber, R
    Bacher, A
    Rohdich, F
    JOURNAL OF MOLECULAR BIOLOGY, 2003, 329 (05) : 973 - 982
  • [40] Isopentenyl diphosphate isomerase: Mechanistic studies of the type-2 enzyme
    Poulter, C. Dale
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2010, 240
12345