ANALYSIS OF BACTERIAL COMMUNITY IN SEDIMENT FROM DALIAN MARINE AREA BY PCR-DGGE

8 marine sediment samples were collected from Dalian marine area and Dandong marine area in China respectively. The bacterial community structures of sediments from Dalian and Dandong marine area were analyzed by denaturing gradient gel electrophoresis (DGGE). Our results showed that the total Gel bands remained relatively stable among all collected sediment samples. The bacterial communities of the sediments could be divided into two groups according to the cluster analysis based on DGGE patterns, and the bacterial community structures were different according to the geographical origins of samples. Moreover, the bacterial communities in sediment samples were consistently different within the different Dalian marine functional zones. The bacterial communities of four mariculture zones were clustered into one sub-group, and conservation district and port area were clustered into another sub-group. Analysis of nucleotide sequence of the dominant bands demonstrated that the major phylogenetic groups identified by DGGE belonged to Proteobacteria, Bacteroidete and Actinobacteria, of which Salinimicrobium sp., Nitrosospira sp. and Methylophaga sp. were the predominant bacteria communities in mariculture zone samples, in addition, Methylophaga sp. were widely distributed in the sediment samples. However, the DGGE gel band which were closely related with uncultured Actinobacteria were detected as one of the predominant species in Y2 samples from Dandong marine sample but not present in other samples.