Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

被引:18
|
作者
Yao, Fang-Jie [1 ,2 ]
Lu, Li-Xin [2 ]
Wang, Peng [2 ]
Fang, Ming [1 ]
Zhang, You-Min [1 ]
Chen, Ying [2 ]
Zhang, Wei-Tong [2 ]
Kong, Xiang-Hui [2 ]
Lu, Jia [1 ]
Honda, Yoichi [3 ]
机构
[1] Jilin Agr Univ, Coll Hort, Changchun, Jilin, Peoples R China
[2] Jilin Agr Univ, Engn Res Ctr, Chinese Minist Educ Edible & Med Fungi, Changchun, Jilin, Peoples R China
[3] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto, Japan
关键词
Auricularia auricula-judae; fruiting body pattern; sequence-related amplified polymorphism; sequence characterized amplified region; SCAR MARKERS; RESISTANCE; MUSHROOM; DISEASE; COLOR;
D O I
10.1080/12298093.2018.1454004
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
引用
收藏
页码:72 / 78
页数:7
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