Germination pretreatment and storage behavior of Terminalia laxiflora seed

Terminalia laxiflora Engl. & Diels. is an important indigenous and multi-purpose species in Ethiopia. However threatened due to low germination and its storage behavior is unknown. In this study, we aimed to (1) test pretreatments for breaking the dormancy of T. laxiflora seed and (2) determine its storage behavior. Seeds were subjected to four pretreatments such as soaking, scarification, high-temperature, and control. Experiments were done before storage and after 2years of dry storage at cold temperature (-10 degrees C) based on randomized design with four replicates. The first round of experimental results showed that T. laxiflora seeds with high temperature treatments at 78 degrees C for 10min showed significantly higher germination percentage (80%). In the second round experiment, high-temperature treatment at 78 degrees C for 15min, cold water soaking, high-temperature treatment at 78 degrees C for 10min, hot water soaking, and high-temperature treatment at 78 degrees C for 5min showed significantly higher germination percentage from the remaining treatments (75, 64, 58, 56, and 53%, respectively). To break the dormancy of T. laxiflora seed and attain good germination result, seeds should be pretreated with high-temperature at 78 degrees C for 10min to test the initial germination before storage and should be pretreated with high-temperature (at 78 degrees C for 15, 10, or 5min), soaked in cold, or hot water for 24h to monitor the germination after storage. The two experiments show that T. laxiflora produces orthodox seed.