Molecular cloning and functional characterization of duck mitochondrial antiviral-signaling protein (MAVS)

Mitochondrial antiviral-signaling protein (MAVS), also called IPS-1/VISA/Cardif, is an important molecule involved in host defense and triggers a signal for producing type I IFN. Currently the function of MAVS in ducks (duMAVS) remains largely unclear while significant progress has been made in mammals. In this study, the full-length duMAVS cDNA was cloned from duck embryo fibroblasts (DEFs) for the first time. Tissue specificity analysis showed duMAVS was universally expressed in all detected tissues. DEFs transfected with duMAVS were able to induce interferon-beta (IFN-beta) expression through activating interferon regulatory factor 1 (IRF1) and nuclear factor kappa B (NF-kappa B). Both the CARD-like domain and transmembrane domain were required for duMAVS signaling via deletion mutant analysis. In addition, poly(I:C)- or Sendai virus (SeV)-induced IFN-beta expression in DEFs were significantly decreased by knockdown of duMAVS with siRNA. Altogether, these results indicate that MAVS is a critical immunoregulator in duck innate immune system. (C) 2015 Elsevier Ltd. All rights reserved.