Detection of enteroviruses from water samples using optimised RT-PCR with universal primers

被引：1
|
作者：
Zhang, C. M. [1 ]
Liu, Y. J. [1 ]
Wang, X. C. [1 ]
Xue, X. P. [2 ]
机构：
[1] Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Peoples R China
[2] Northwestern Polytech Univ, Fac Life Sci, Xian 710072, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENT AND POLLUTION | 2009年 / 38卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
enteroviruses; universal primers; RT-PCR; simultaneous detection; concentration; microporous filter; HEPATITIS-A VIRUS; ENTERIC VIRUSES; INFECTIOUS ENTEROVIRUSES; PATHOGENIC VIRUSES; ROTAVIRUS; SEWAGE; IDENTIFICATION; CALIFORNIA; POLIOVIRUS;
D O I：
10.1504/IJEP.2009.026658
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A method for rapid detection of enteroviruses in water sample was established by using RT-PCR with universal primers with PV1, PV2, PV3 and CVB3 as the reference viruses. The conditions for simultaneous detection of different enteroviruses were experimentally determined and the detection limit of virus was found to be 38CCID(50). Virus concentration, RNA extraction and RT-PCR operation were the main factors affecting the sensitivity of virus detection. By applying the procedures to water samples with seeded PV1 viruses, the interference of impurities in practical waters on enteroviruses detection was investigated. The method was proved to be suitable for practical application.
引用
收藏
页码：151 / 165
页数：15
相关论文
共 50 条
  • [1] Simultaneous detection of enteroviruses from surface waters by real-time RT-PCR with universal primers
    Chongmiao Zhang Xiaochang Wang Yongjun Liu Dangcong Peng Key Laboratory of Northwest Water Resource Ecology and Environment Ministry of Education School of Environmental and Municipal Engineering Xian University of Architecture and Technology Xian China Postdoctoral Research Center of Civil Engineering Xian University of Architecture and Technology Xian China Engineering Research Center for Wastewater Treatment and Reclamation of Shaanxi Province Xian China
    Journal of Environmental Sciences, 2010, 22 (08) : 1261 - 1266
  • [2] Simultaneous detection of enteroviruses from surface waters by real-time RT-PCR with universal primers
    Zhang, Chongmiao
    Wang, Xiaochang
    Liu, Yongjun
    Peng, Dangcong
    JOURNAL OF ENVIRONMENTAL SCIENCES, 2010, 22 (08) : 1261 - 1266
  • [3] Simultaneous detection of enteroviruses from surface waters by real-time RT-PCR with universal primers
    Chongmiao Zhang 1
    Journal of Environmental Sciences, 2010, (08) : 1261 - 1266
  • [4] Development of universal primers for detection of potato carlaviruses by RT-PCR
    Nie, Xianzhou
    Bai, Yanju
    Molen, Teresa A.
    Desjardins, David C.
    JOURNAL OF VIROLOGICAL METHODS, 2008, 149 (02) : 209 - 216
  • [5] RT-PCR and chemiluminescent ELISA for detection of enteroviruses
    Greening, GE
    Woodfield, L
    Lewis, GD
    JOURNAL OF VIROLOGICAL METHODS, 1999, 82 (02) : 157 - 166
  • [6] Molecular detection and characterization of human enteroviruses directly from clinical samples using RT-PCR and DNA Sequencing
    Iturriza-Gómara, M
    Megson, B
    Gray, J
    JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (02) : 243 - 253
  • [7] Detection of dengue virus by simple RT-PCR using universal degenerate primers Observations from a preliminary study
    Suwanwong, Y.
    Moungkote, T.
    Wiwanitkit, V.
    Soogarun, S.
    ARCHIVES OF HELLENIC MEDICINE, 2010, 27 (05): : 818 - 821
  • [8] A comparison of two sets of primers for the RT-PCR detection of astroviruses in environmental samples
    Marx, FE
    Taylor, MB
    Grabow, WOK
    WATER SA, 1997, 23 (03) : 257 - 262
  • [9] Detection of enteroviruses and hepatitis a virus in water by consensus primer multiplex RT-PCR
    Li, JW
    Wang, XW
    Yuan, CQ
    Zheng, JL
    Jin, M
    Song, N
    Shi, XQ
    Chao, FH
    WORLD JOURNAL OF GASTROENTEROLOGY, 2002, 8 (04) : 699 - 702
  • [10] Detection of enteroviruses and hepatitis a virus in water by consensus primer multiplex RT-PCR
    Jun-Wen Li Xin-Wei Wang Chang-Qing Yuan Jin-Lai Zheng Min Jin Nong Song Xiu-Quan Shi Fu-Huan Chao Department of Environment and Health
    World Journal of Gastroenterology, 2002, 8 (04) : 699 - 702
12345