LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1

被引：45
|
作者：
Liu, Yao [1 ,2 ]
Wang, Li-Li [3 ]
Chen, Shuo [3 ]
Zong, Zhi-Hong [4 ]
Guan, Xue [3 ]
Zhao, Yang [1 ,2 ]
机构：
[1] Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Dept Obstet & Gynecol, Guangzhou 510150, Guangdong, Peoples R China
[2] Guangdong Higher Educ Inst Guangdong Prov, Key Lab Reprod & Genet, Guangzhou 510150, Guangdong, Peoples R China
[3] China Med Univ, Affiliated Hosp 1, Dept Gynecol, Shenyang 110001, Liaoning, Peoples R China
[4] China Med Univ, Coll Basic Med, Dept Biochem & Mol Biol, Shenyang 100013, Liaoning, Peoples R China
来源：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2018年 / 22卷 / 08期
基金：
中国国家自然科学基金;
关键词：
cyclin D1; endometrial carcinoma; LncRNA ABHD11-AS1; tumorigenesis and progression; INHIBITS CELL-PROLIFERATION; LONG NONCODING RNAS; PTEN EXPRESSION; BETA-CATENIN; CANCER; POLYMORPHISM;
D O I：
10.1111/jcmm.13675
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To investigate the expression, role and mechanism of action of long non-coding RNA (lncRNA) ABHD11-AS1 in endometrial carcinoma. The expression of lncRNA ABHD11-AS1 was quantified by qRT-PCR in human endometrial carcinoma (n=89) and normal endometrial tissues (n=27). LncRNA ABHD11-AS1 was stably overexpressed or knocked-down in endometrial carcinoma cell lines to examine the cellular phenotype and expression of related molecules. Compared to normal endometrial tissue, lncRNA ABHD11-AS1 was significantly overexpressed in endometrial carcinoma. Overexpression of lncRNA ABHD11-AS1 promoted the proliferation, G1-S progression, invasion and migration of endometrial cancer cells; inhibited apoptosis; up-regulated cyclin D1, CDK1, CDK2, CDK4, Bcl-xl and VEGFA; and down-regulated p16, while ABHD11-AS1 down-regulation has the opposite effect. RNA pull down demonstrated that lncRNA ABHD11-AS1 binds directly to cyclin D1. Knockdown of cyclin D1 can reverse the effect of ABHD11-AS1. Overexpression of lncRNA ABHD11-AS1 increased the tumorigenicity and up-regulated cyclin D1 in an invivo model of endometrial cancer in nude mice. LncRNA ABHD11-AS1 functions as an oncogene to promote cell proliferation and invasion in endometrial carcinoma by positively targeting cyclin D1.
引用
收藏
页码：3955 / 3964
页数：10
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