Identification of Epilobium species and willow-herbs (Onagraceae) by HPLC analysis of flavonoids as chemotaxonomic markers

被引:10
|
作者
Toth, B. Hevesi [1 ]
Balazs, A. [1 ]
Vukics, V. [1 ]
Szoke, E. [1 ]
Kery, A. [1 ]
机构
[1] Semmelweis Univ, Dept Pharmacognosy, H-1085 Budapest, Hungary
关键词
column liquid chromatography; epilobium species; willow-herbs flavonoid composition; myricitrin;
D O I
10.1365/s10337-006-0740-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this work was to compare macro- and micromorphological characteristics and flavonoid composition of some Epilobium species and commercial willow-herbs for their authentication and quality control. In order to determine the flavonoid composition of extracts, the flavonol-glycosides were also hydrolised quantitatively to their aglycones. Before and after hydrolysis flavonoid fingerprints were studied by high-performance liquid chromatography using two different gradient mobile phase systems. Flavonoid composition was found to be suitable for the authentication of Epilobium species and commercial willow-herbs as an alternative to the current phytochemical study and time-consuming microscopic procedures [1]. The combined HPLC analysis of free flavonoid-aglycones and glycosides, developed in our laboratory can be used for the quality control of willow-herb samples and various commercial preparations.
引用
收藏
页码:S119 / S123
页数:5
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