Two-fraction and three-fraction continuous simulated moving bed separation of nucleosides

被引：49

作者：

Abel, S ^{[1
]}

Bäbler, MU ^{[1
]}

Arpagaus, C ^{[1
]}

Mazzotti, M ^{[1
]}

Stadler, J ^{[1
]}

机构：

[1] ETH, Swiss Fed Inst Technol, Inst Proc Engn, CH-8092 Zurich, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1043卷 / 02期

关键词：

simulated moving bed; preparative chromatography; nucleosides;

D O I：

10.1016/j.chroma.2004.05.094

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new experimental set-up and a new simulated moving bed (SMB) operation are presented in this work. A desktop SMB unit developed as a modification of the commercial AKTA(TM) explorer working platform has been utilized for the separation of different mixtures of nucleosides. Both two fraction and three fraction SMB separations have been carried out, the latter made possible by the adoption of a new SMB configuration and operating mode (three fraction SMB, 3F-SMB, operation). Experiments demonstrate the feasibility of the 3F-SMB operation, and confirm the trends predicted based on considerations about retention of the components to be separated along the unit. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：201 / 210

页数：10

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