Spatial distribution of centromeres and telomeres at interphase varies among Brachypodium species

被引:16
|
作者
Idziak, Dominika [1 ]
Robaszkiewicz, Ewa [1 ]
Hasterok, Robert [1 ]
机构
[1] Silesian Univ, Fac Biol & Environm Protect, Dept Plant Anat & Cytol, PL-40032 Katowice, Poland
关键词
Brachypodium; centromeres; interphase; model grass; molecular cytogenetics; Rabl configuration; telomeres; ORDER CHROMATIN ARRANGEMENTS; NUCLEAR-DNA CONTENT; ART; NO; 44; CHROMOSOME TERRITORIES; MODEL GRASS; ARABIDOPSIS-THALIANA; GENOME ORGANIZATION; FISSION YEAST; DROSOPHILA-MELANOGASTER; RABL CONFIGURATION;
D O I
10.1093/jxb/erv369
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this study the 3-D distribution of centromeres and telomeres was analysed in the interphase nuclei of three Brachypodium species, i.e. B. distachyon (2n= 10), B. stacei (2n= 20) and B. hybridum (2n= 30), which is presumably a hybrid between the first two species. Using fluorescence in situ hybridization (FISH) with centromeric and telomeric DNA probes, it was observed that the majority of B. distachyon nuclei in the root tip cells displayed the Rabl configuration while both B. stacei and B. hybridum mostly lacked the centromere-telomere polarization. In addition, differentiated leaf cells of B. distachyon did not display the Rabl pattern. In order to analyse the possible connection between the occurrence of the Rabl pattern and the phase of cell cycle or DNA content, FISH was combined with digital image cytometry. The results revealed that the frequency of nuclei with the Rabl configuration in the root tip nuclei was positively correlated with an increase in DNA content, which resulted from DNA replication. Also, the analysis of the influence of the nuclear shape on the nuclear architecture indicated that an increasing elongation of the nuclei negatively affected the occurrence of the Rabl pattern. Some possible explanations of these phenomena are discussed.
引用
收藏
页码:6623 / 6634
页数:12
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