The indigo carmine was reduced by using cheap, easily attainable and non-poisonous reagents. Therefore, a special reagent was developed to measure the concentration of dissolved oxygen in water in situ. The molar absorption coefficient of the method is 1.35x10(4), and its dynamic linear range is 0-27 mg/L linearity coefficient of 0.997. The most oxidizing species, such as Cl-2, ClO-, Cu2+, Fe3+, V5+, and As( V), do not interfere the determination. It takes no more than 1 min to analyze one sample. The method was applied to determining some practical samples and all the results obtained were satisfactory.