Fluorometric assay for determining epoxide hydrolase activity

被引：16
|
作者：
Doderer, K [1 ]
Schmid, RD [1 ]
机构：
[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 10期
关键词：
carboxyfluoroescein; epoxide hydrolase; fluorometric assay; sodium periodate;
D O I：
10.1023/B:BILE.0000025887.36874.33
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescein.
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页码：835 / 839
页数：5
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