Fluorometric assay for determining epoxide hydrolase activity

被引：16
|
作者：
Doderer, K [1 ]
Schmid, RD [1 ]
机构：
[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 10期
关键词：
carboxyfluoroescein; epoxide hydrolase; fluorometric assay; sodium periodate;
D O I：
10.1023/B:BILE.0000025887.36874.33
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescein.
引用
收藏
页码：835 / 839
页数：5
相关论文
共 50 条
  • [1] Fluorometric assay for determining epoxide hydrolase activity
    Kai Doderer
    Rolf D. Schmid
    [J]. Biotechnology Letters, 2004, 26 : 835 - 839
  • [2] CONTINUOUS FLUOROMETRIC ASSAY OF EPOXIDE HYDRASE ACTIVITY
    DANSETTE, PM
    DUBOIS, GC
    JERINA, DM
    [J]. ANALYTICAL BIOCHEMISTRY, 1979, 97 (02) : 340 - 345
  • [3] Spectrophotometric assay for epoxide hydrolase activity toward any epoxide
    Doderer, K
    Lutz-Wahl, S
    Hauer, B
    Schmid, RD
    [J]. ANALYTICAL BIOCHEMISTRY, 2003, 321 (01) : 131 - 134
  • [4] A spectrophotometric method to assay epoxide hydrolase activity
    Bhatnagar, T
    Manoj, KM
    Baratti, JC
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 50 (01): : 1 - 13
  • [5] A colorimetric assay suitable for screening epoxide hydrolase activity
    Zocher, F
    Enzelberger, MM
    Bornscheuer, UT
    Hauer, B
    Schmid, RD
    [J]. ANALYTICA CHIMICA ACTA, 1999, 391 (03) : 345 - 351
  • [6] A spectrophotometric assay for measuring and detecting an epoxide hydrolase activity
    Mateo, C
    Archelas, A
    Furstoss, R
    [J]. ANALYTICAL BIOCHEMISTRY, 2003, 314 (01) : 135 - 141
  • [7] A SENSITIVE FLUOROMETRIC ASSAY FOR EPOXIDE HYDRATASE
    HUKKELHOVEN, MWAC
    VROMANS, EWM
    VERMORKEN, AJM
    BLOEMENDAL, H
    [J]. FEBS LETTERS, 1982, 144 (01) : 104 - 108
  • [8] FLUOROMETRIC ASSAY FOR PANCREATIC CHOLESTERYLESTER HYDROLASE
    JOUTTI, A
    KOTAMA, L
    VIRTANEN, JA
    KINNUNEN, PKJ
    [J]. CHEMISTRY AND PHYSICS OF LIPIDS, 1985, 36 (04) : 335 - 341
  • [9] Data set of optimal parameters for colorimetric red assay of epoxide hydrolase activity
    deOliveira, Gabriel Stephani
    Adriani, Patricia Pereira
    Borges, Flavia Garcia
    Lopes, Adriana Rios
    Campana, Patricia T.
    Chambergo, Felipe S.
    [J]. DATA IN BRIEF, 2016, 8 : 436 - 440
  • [10] PHOTOMETRIC ASSAY OF HEPATIC EPOXIDE HYDROLASE ACTIVITY WITH SAFROLE OXIDE (SAFO) AS SUBSTRATE
    WATABE, T
    AKAMATSU, K
    [J]. BIOCHEMICAL PHARMACOLOGY, 1974, 23 (20) : 2839 - 2844
12345