Crystallization and preliminary crystallographic studies of the Pasteurella multocida toxin catalytic domain

被引:4
|
作者
Miyazawa, Masayuki
Kitadokoro, Kengo
Kamitani, Shigeki
Shime, Hiroaki
Horiguchi, Yasuhiko
机构
[1] Osaka Univ, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan
[2] Kyoto Univ, Res Ctr Low Temp & Mat Sci, Sakyo Ku, Kyoto 6068502, Japan
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷
关键词
DERMONECROTIC TOXIN; MITOGENIC TOXIN; POTENT MITOGEN; BORDETELLA-BRONCHISEPTICA; SWISS; 3T3-CELLS; BONE-RESORPTION; PROTEIN-KINASE; SEQUENCE; GROWTH; CELLS;
D O I
10.1107/S1744309106030375
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The C-terminal catalytic domain of Pasteurella multocida toxin, which is the virulence factor of the organism in P. multocida, has been expressed, purified and subsequently crystallized using the sitting-drop vapour-diffusion technique. Native diffraction data to 1.9 angstrom resolution were obtained at the BL44XU beamline of SPring-8 from a flash-frozen crystal at 100 K. The crystals belong to space group C2, with unit-cell parameters a = 111.0, b = 150.4, c = 77.1 angstrom, beta = 105.5 degrees, and are likely to contain one C-PMT (726 residues) per asymmetric unit.
引用
收藏
页码:906 / 908
页数:3
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