Crystallization and preliminary crystallographic studies of the Pasteurella multocida toxin catalytic domain

The C-terminal catalytic domain of Pasteurella multocida toxin, which is the virulence factor of the organism in P. multocida, has been expressed, purified and subsequently crystallized using the sitting-drop vapour-diffusion technique. Native diffraction data to 1.9 angstrom resolution were obtained at the BL44XU beamline of SPring-8 from a flash-frozen crystal at 100 K. The crystals belong to space group C2, with unit-cell parameters a = 111.0, b = 150.4, c = 77.1 angstrom, beta = 105.5 degrees, and are likely to contain one C-PMT (726 residues) per asymmetric unit.