Micellar electrokinetic capillary chromatographic method for simultaneous determination of drugs used to treat advanced breast cancer

A micellor electrokinetic capillary chromatography (MEKC) method has been developed for quantification of four drugs - tamoxifen, anastrozole, letrozole, and methotrexate - used to treat advanced breast cancer. Separation was performed at 25 degreesC and 25 kV, with 20 mm borate buffer (pH 9.2) containing 40 mm sodium cloclecylsulfate as electrolyte solution. Under these conditions analyses were performed in 12 min. The linearity of the response was investigated for the concentration range 2.0 - 20.0 mg L-1. The intra-day residual standard deviation (n = 4 graphs) between the slopes of the calibration graphs was acceptable for the four drugs studied. Detection limits (signal-to-noise ratio = 3) were below 1 mg L-1 for all the compounds. The simplicity; precision, and sensitivity of MEKC proved suitable for quality control of pharmaceutical preparations used to treat advanced breast cancer Six different pharmaceutical preparations, each containing one of the above-mentioned drugs, were successfully analyzed.