Comparison of 'real-time' and immunometric RT-PCR: RNA interference of reverse transcriptase-PCR

被引：1

作者：

Hoadley, ME ^{[1
]}

Hopkins, SJ ^{[1
]}

机构：

[1] Univ Manchester, Hope Hosp, Div Med & Neurosci, Salford M6 8HD, Lancs, England

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2006年 / 312卷 / 1-2期

关键词：

RT-PCR; mRNA; 'real-time';

D O I：

10.1016/j.jim.2006.02.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We measured interleukin-6 (IL-6) mRNA in whole blood, using an immunometric reverse transcriptase-polymerase chain reaction (IRT-PCR) method and 'real-time' RT-PCR using the Roche LightCycler. Both methods showed similar precision, in terms of inter- and intra-assay variation, where a lower total RNA concentration was used at a relatively high ratio of (IL-6) mRNA to total RNA. However, the 'real-time' RT-PCR method showed a greater loss of accuracy at higher concentrations of total RNA. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：40 / 44

页数：5

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