Single-molecule Imaging with an Inexpensive UV-LED Light Source

被引：6
|
作者：
Hattori, Akifumi [1 ]
Habuchi, Satoshi [1 ]
Vacha, Martin [1 ]
机构：
[1] Tokyo Inst Technol, Dept Organ & Polymer Mat, Meguro Ku, Tokyo 1528552, Japan
来源：
CHEMISTRY LETTERS | 2009年 / 38卷 / 03期
关键词：
MICROSCOPY; SPECTROSCOPY; ENSEMBLE;
D O I：
10.1246/cl.2009.234
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The powerful tool of single-molecule detection and spectroscopy has so far been confined mainly to the visible spectral region. Here, we report the use of a low-cost high-power UV light emitting diode (LED) emitting at 365 nm as a light source for single-molecule detection in epi-fluorescence microscopy. The LED provides an affordable alternative to UV laser systems. The performance of the light source is demonstrated by recording fluorescence images and time-dependent fluorescence traces of single molecules of a laser dye p-bis(o-methylstyryl)benzene (bis-MSB) and of a conjugated polymer polyfluorene.
引用
收藏
页码：234 / 235
页数：2
相关论文
共 50 条
  • [21] Light-Induced Subnanometric Modulation of a Single-Molecule Electron Source
    Yanagisawa, Hirofumi
    Bohn, Markus
    Kitoh-Nishioka, Hirotaka
    Goschin, Florian
    Kling, Matthias F.
    PHYSICAL REVIEW LETTERS, 2023, 130 (10)
  • [22] The Impact of Unfiltered Source Water Characteristics on UV-LED Disinfection Efficiency
    Makuei, Mehrnaz Soleimanpour
    Peleato, Nicolas
    PROCEEDINGS OF THE CANADIAN SOCIETY FOR CIVIL ENGINEERING ANNUAL CONFERENCE 2023, VOL 8, CSCE 2023, 2024, 502 : 249 - 258
  • [23] A deep-curing UV-LED light photoinitiator based on diphenylpropanetrione
    Wang, Xuewei
    Li, Wenjie
    Nie, Jun
    Zhu, Xiaoqun
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2024, 454
  • [24] Single-Molecule Light-Sheet Imaging of Suspended T Cells
    Ponjavic, Aleks
    McColl, James
    Carr, Alexander R.
    Santos, Ana Mafalda
    Kulenkampff, Klara
    Lippert, Anna
    Davis, Simon J.
    Klenerman, David
    Lee, Steven F.
    BIOPHYSICAL JOURNAL, 2018, 114 (09) : 2200 - 2211
  • [25] White light generation from CdS nanoparticles illuminated by UV-LED
    Suhail, A. M.
    Khalifa, M. J.
    Saeed, N. M.
    Ibrahim, O. A.
    EUROPEAN PHYSICAL JOURNAL-APPLIED PHYSICS, 2010, 49 (03):
  • [26] A new UV source activates ozone for water treatment: Wavelength-dependent ultraviolet light-emitting diode (UV-LED)
    Wang, Jie
    Liu, Haibao
    Wang, Yan
    Ma, Defang
    Yao, Guangping
    Yue, Qinyan
    Gao, Baoyu
    Xu, Xing
    SEPARATION AND PURIFICATION TECHNOLOGY, 2022, 280
  • [27] MICROSCOPY Single-molecule light absorption
    Hofkens, Johan
    Roeffaers, Maarten B. J.
    NATURE PHOTONICS, 2011, 5 (02) : 80 - 81
  • [28] Development and future of ultraviolet light-emitting diodes: UV-LED will replace the UV lamp
    Muramoto, Yoshihiko
    Kimura, Masahiro
    Nouda, Suguru
    SEMICONDUCTOR SCIENCE AND TECHNOLOGY, 2014, 29 (08)
  • [29] Single-molecule imaging in catalytic polymerization
    Blum, Suzanne
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 256
  • [30] Novel fluorophores for single-molecule imaging
    1600, American Chemical Society (125):
12345