Single-molecule Imaging with an Inexpensive UV-LED Light Source

被引：6
|
作者：
Hattori, Akifumi [1 ]
Habuchi, Satoshi [1 ]
Vacha, Martin [1 ]
机构：
[1] Tokyo Inst Technol, Dept Organ & Polymer Mat, Meguro Ku, Tokyo 1528552, Japan
来源：
CHEMISTRY LETTERS | 2009年 / 38卷 / 03期
关键词：
MICROSCOPY; SPECTROSCOPY; ENSEMBLE;
D O I：
10.1246/cl.2009.234
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The powerful tool of single-molecule detection and spectroscopy has so far been confined mainly to the visible spectral region. Here, we report the use of a low-cost high-power UV light emitting diode (LED) emitting at 365 nm as a light source for single-molecule detection in epi-fluorescence microscopy. The LED provides an affordable alternative to UV laser systems. The performance of the light source is demonstrated by recording fluorescence images and time-dependent fluorescence traces of single molecules of a laser dye p-bis(o-methylstyryl)benzene (bis-MSB) and of a conjugated polymer polyfluorene.
引用
收藏
页码：234 / 235
页数：2
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