EFFECTS OF PROBIOTIC ON MORPHOLOGICAL CHANGES IN PORCINE MACROPHAGES DURING IN VITRO CULTIVATION

Nowadays, Bifidobacterium bifidum, Lactobacillus rhamnosus and Enterococcus faecium are frequently used probiotics in porcine nutrition. The probiotics-immunobiotics positively influence function of gastrointestinal tract and can also modulate function of immune system. The probiotics interact with immune cells as macrophages, neutrophils, dendritic cells or other immune cells and stimulate them to produce cytokines or to enhance phagocytosis. The aim of this study was to determine whether interactions of probiotics with porcine monocyte derived macrophages (MDMF) lead to structural changes of these cells during in vitro cultivation. We used the light microscopy and our findings suggest that probiotics affected the structural changes of porcine MDMF. Part of MDMF underwent apoptosis or necrosis and it was described the different stadia leading to the cell death. In some MDMF numerous vacuoles are accumulated in cytoplasm. The most pronounced structural changes of MDMF were caused by Enterococcus faecium. Finally, interactions of probiotics with MDMF were associated with phagocytosis all used probiotics.