Trogocytosis of MHC-I/Peptide Complexes Derived from Tumors and Infected Cells Enhances Dendritic Cell Cross-Priming and Promotes Adaptive T Cell Responses

被引:42
|
作者
Zhang, Qian-Jin [1 ]
Li, Xiao-Lin [1 ]
Wang, David [1 ]
Huang, Xiao-Cong [1 ]
Mathis, J. Michael [1 ]
Duan, Wei-Ming [1 ]
Knight, David [1 ]
Shi, Runhua [1 ]
Glass, Jonathan [1 ]
Zhang, Dong-Qing [2 ]
Eisenbach, Lea [3 ]
Jefferies, Wilfred A. [4 ]
机构
[1] Louisiana State Univ, Hlth Sci Ctr, Feist Weiller Canc Ctr, Dept Cellular Biol & Anat,Gene Therapy Program, Shreveport, LA 71105 USA
[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai, Peoples R China
[3] Weizmann Inst Sci, Dept Immunol, Rehovot, Israel
[4] Univ British Columbia, Dept Med Genet, Microbiol & Immunol & Zool, Biomed Res Ctr, Michael Smith Lab, Vancouver, BC V5Z 1M9, Canada
来源
PLOS ONE | 2008年 / 3卷 / 08期
基金
中国国家自然科学基金;
关键词
D O I
10.1371/journal.pone.0003097
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The transporter associated with antigen processing ( TAP) and the major histocompatibility complex class I (MHC-I), two important components of the MHC-I antigen presentation pathway, are often deficient in tumor cells. The restoration of their expression has been shown to restore the antigenicity and immunogenicity of tumor cells. However, it is unclear whether TAP and MHC-I expression in tumor cells can affect the induction phase of the T cell response. To address this issue, we expressed viral antigens in tumors that are either deficient or proficient in TAP and MHC-I expression. The relative efficiency of direct immunization or immunization through cross-presentation in promoting adaptive T cell responses was compared. The results demonstrated that stimulation of animals with TAP and MHC-I proficient tumor cells generated antigen specific T cells with greater killing activities than those of TAP and MHC-I deficient tumor cells. This discrepancy was traced to differences in the ability of dendritic cells (DCs) to access and sample different antigen reservoirs in TAP and MHC-I proficient versus deficient cells and thereby stimulate adaptive immune responses through the process of cross-presentation. In addition, our data suggest that the increased activity of T cells is caused by the enhanced DC uptake and utilization of MHC-I/peptide complexes from the proficient cells as an additional source of processed antigen. Furthermore, we demonstrate that immune-escape and metastasis are promoted in the absence of this DC 'arming' mechanism. Physiologically, this novel form of DC antigen sampling resembles trogocytosis, and acts to enhance T cell priming and increase the efficacy of adaptive immune responses against tumors and infectious pathogens.
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页数:14
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