Production of trehalose from starch by novel trehalose-producing enzymes from Sulfolobus solfataricus KM1

被引：30

作者：

Kobayashi, K ^{[1
]}

Komeda, T ^{[1
]}

Miura, Y ^{[1
]}

Kettoku, M ^{[1
]}

Kato, M ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 83卷 / 03期

关键词：

trehalose; trehalose-producing enzymes; starch; Sulfolobus solfataricus;

D O I：

10.1016/S0922-338X(97)80996-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new process for trehalose production from starch was developed using a novel glycosyltransferase and a novel alpha-amylase from Sulfolobus solfataricus KM1. The yield of trehalose from starch was 81.5% using the two enzymes and a thermostable debranching enzyme. Trehalose production was carried out at high temperature over 60 degrees C and at a high concentration of starch with no risk of contamination bg microorganisms or retrogradation of starch.

引用

页码：296 / 298

页数：3

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