Production of trehalose from starch by novel trehalose-producing enzymes from Sulfolobus solfataricus KM1

被引：30
|
作者：
Kobayashi, K [1 ]
Komeda, T [1 ]
Miura, Y [1 ]
Kettoku, M [1 ]
Kato, M [1 ]
机构：
[1] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 83卷 / 03期
关键词：
trehalose; trehalose-producing enzymes; starch; Sulfolobus solfataricus;
D O I：
10.1016/S0922-338X(97)80996-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new process for trehalose production from starch was developed using a novel glycosyltransferase and a novel alpha-amylase from Sulfolobus solfataricus KM1. The yield of trehalose from starch was 81.5% using the two enzymes and a thermostable debranching enzyme. Trehalose production was carried out at high temperature over 60 degrees C and at a high concentration of starch with no risk of contamination bg microorganisms or retrogradation of starch.
引用
收藏
页码：296 / 298
页数：3
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