Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG′::lux

被引：10
|
作者：
Tienaho, Jenni [1 ,2 ]
Sarjala, Tytti [2 ]
Franzen, Robert [1 ]
Karp, Matti [1 ]
机构：
[1] Tampere Univ Technol, Dept Chem & Bioengn, FI-33101 Tampere, Finland
[2] Nat Resources Inst Finland Luke, Parkano Res Unit, FI-39700 Parkano, Finland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 118卷
关键词：
Antioxidative activity; Bacterial biosensor; Bioscreening; Microplate technique; ASSAY;
D O I：
10.1016/j.mimet.2015.08.018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：78 / 80
页数：3
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