Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae

被引：4
|
作者：
Mohd-Sharif, Nurhikmah [1 ]
Shaibullah, Sofiyah [1 ]
Givajothi, Vasanthakumar [2 ]
Tan, Cheng-Seng [2 ]
Ho, Kok Lian [3 ]
Teh, Aik-Hong [4 ]
Baharum, Syarul Nataqain [1 ]
Waterman, Jitka [5 ]
Ng, Chyan Leong [1 ]
机构：
[1] Univ Kebangsaan Malaysia, Inst Syst Biol, Ukm Bangi 43600, Selangor, Malaysia
[2] Univ Kebangsaan Malaysia, Sch Biosci & Biotechnol, Fac Sci & Technol, Ukm Bangi 43600, Selangor, Malaysia
[3] Univ Putra Malaysia, Dept Pathol, Fac Med & Hlth Sci, Upm Serdang 43400, Selangor, Malaysia
[4] Univ Sains Malaysia, Ctr Chem Biol, 10 Persiaran Bukit Jambul, Bayan Lepas 11900, Penang, Malaysia
[5] Diamond Light Source, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2017年 / 73卷
关键词：
Streptomyces fradiae; recombinantTylP protein; gamma-butyrolactone; GBL; transcription factors; tylosin; AUTOREGULATOR RECEPTOR; TETR FAMILY; A-FACTOR; SECONDARY METABOLISM; FUNCTIONAL-ANALYSIS; TRANSCRIPTIONAL REGULATORS; MOLECULAR REPLACEMENT; MICROBIAL HORMONE; COELICOLOR A3(2); STRUCTURAL BASIS;
D O I：
10.1107/S2053230X17001212
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
TylP is one of five regulatory proteins involved in the regulation of antibiotic (tylosin) production, morphological and physiological differentiation in Streptomyces fradiae. Its function is similar to those of various gamma-butyrolactone receptor proteins. In this report, N-terminally His-tagged recombinant TylP protein (rTylP) was overproduced in Escherichia coli and purified to homogeneity. The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X-rays to 3.05 angstrom resolution and belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 126.62, c = 95.63 angstrom.
引用
收藏
页码：109 / 115
页数：7
相关论文
共 50 条
  • [41] Crystallization and preliminary X-ray crystallographic analysis of human autotaxin
    Inoue, Keigo
    Tanaka, Nobutada
    Haga, Arayo
    Yamasaki, Kyohei
    Umeda, Tomonobu
    Kusakabe, Yoshio
    Sakamoto, Yasumitsu
    Nonaka, Takamasa
    Deyashiki, Yoshihiro
    Nakamura, Kazuo T.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 450 - 453
  • [42] Crystallization and preliminary X-ray analysis of recombinant staphylokinase
    Rabijns, A
    Baeyens, K
    DeBondt, HL
    DeRanter, C
    PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1997, 27 (01): : 160 - 161
  • [43] Mosquito-larvicidal binary toxin receptor protein (Cqm1): crystallization and X-ray crystallographic analysis
    Sharma, Mahima
    Lakshmi, Ashwitha
    Gupta, Gagan D.
    Kumar, Vinay
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2018, 74 : 571 - 577
  • [44] Crystallization and preliminary X-ray crystallographic studies of human FAIM protein
    Gu, Jiang
    Ji, Xiaowei
    Qi, Jianxun
    Ma, Ying
    Mao, Xuhu
    Zou, Quanming
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 929 - 931
  • [45] Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus
    Fislage, Marcus
    Roovers, Martine
    Munnich, Stefan
    Droogmans, Louis
    Versees, Wim
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1432 - 1435
  • [46] Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein
    Kang, Wonchull
    Yang, Jin Kuk
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2012, 68 : 1518 - 1520
  • [47] Crystallization and preliminary X-ray crystallographic analysis of rat calcineurin B homologous protein 1
    Naoe, Y
    Arita, K
    Hashimoto, H
    Kanazawa, H
    Sato, M
    Shimizu, T
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 612 - 613
  • [48] Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1
    Bong, Seoung Min
    Yang, Seung Won
    Choi, Ji-Woong
    Kim, Seung Jun
    Lee, Byung Il
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 : 261 - 265
  • [49] Crystallization and preliminary X-ray crystallographic analysis of orotate phosphoribosyltransferase from Helicobacter pylori
    Kim, MK
    Song, HE
    Kim, YS
    Rho, SH
    Im, YJ
    Lee, JH
    Kang, GB
    Eom, SH
    MOLECULES AND CELLS, 2003, 15 (03) : 361 - 363
  • [50] Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the mushroom Agaricus bisporus
    Ismaya, Wangsa T.
    Rozeboom, Henriette J.
    Schurink, Marloes
    Boeriu, Carmen G.
    Wichers, Harry
    Dijkstra, Bauke W.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 575 - 578
12345