Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups

被引:20
|
作者
Fraisier, Christophe [1 ]
Stor, Richard [1 ]
Tenebray, Bernard [1 ]
Sanson, Yannick [1 ]
Nicolas, Pierre [1 ]
机构
[1] IMTSSA, Unite Meningocoque, IRBA, WHO,Collaborating Ctr, F-13262 Marseille 07, France
关键词
MENINGOCOCCAL MENINGITIS; IDENTIFICATION; NIGER; CAPSULE; DISEASE;
D O I
10.1128/JCM.02415-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.
引用
收藏
页码:2662 / 2666
页数:5
相关论文
共 40 条
  • [21] Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application
    Jiang, Yixiang
    Fang, Lin
    Shi, Xiaolu
    Zhang, Hailong
    Li, Yinghui
    Lin, Yiman
    Qiu, Yaqun
    Chen, Qingliang
    Li, Hui
    Zhou, Li
    Hu, Qinghua
    JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (04) : 1266 - 1268
  • [22] New multiplex PCR-based protocol allowing indirect diagnosis of FSHD on single cells: can PGD be offered despite high risk of recombination?
    Barat-Houari, Mouna
    Nguyen, Karine
    Bernard, Rafaelle
    Fernandez, Celine
    Vovan, Catherine
    Bareil, Corinne
    Van Kien, Philippe Khau
    Thorel, Delphine
    Tuffery-Giraud, Sylvie
    Vasseur, Francis
    Attarian, Shahram
    Pouget, Jean
    Girardet, Anne
    Levy, Nicolas
    Claustres, Mireille
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2010, 18 (05) : 533 - 538
  • [23] New multiplex PCR-based protocol allowing indirect diagnosis of FSHD on single cells: can PGD be offered despite high risk of recombination?
    Mouna Barat-Houari
    Karine Nguyen
    Rafaëlle Bernard
    Céline Fernandez
    Catherine Vovan
    Corinne Bareil
    Philippe Khau Van Kien
    Delphine Thorel
    Sylvie Tuffery-Giraud
    Francis Vasseur
    Shahram Attarian
    Jean Pouget
    Anne Girardet
    Nicolas Lévy
    Mireille Claustres
    European Journal of Human Genetics, 2010, 18 : 533 - 538
  • [24] Simultaneous measurement of the antibody responses against SARS-CoV-2 and its multiple variants by a phage display mediated immuno-multiplex quantitative PCR-based assay
    Chen, Hanyi
    Li, Shen
    Wang, Jiali
    He, Siqi
    Wang, Dong
    Qian, Zhaohui
    Hu, Dandan
    Qi, Fangfang
    Hu, Keping
    Luo, Chenyi
    Wang, Jianxun
    FRONTIERS IN MICROBIOLOGY, 2022, 13
  • [25] Development and Characterization an NGS Myeloid Panel: A Single-Tube, Multiplex-PCR-Based NGS Assay with 739 Tiled Amplicons
    Lodato, N. J.
    Liu, Z.
    Kontor, A.
    Polvino, S.
    Ke, Y.
    Wright, W.
    Wang, Z.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2019, 21 (06): : 1149 - 1150
  • [26] A multiplex nested-PCR assay for sensitive and simultaneous detection and direct identification of phytoplasma in the Elm yellows group and Stolbur group and its use in survey of grapevine yellows in France
    Clair, D
    Larrue, J
    Aubert, G
    Gillet, J
    Cloquemin, G
    Boudon-Padieu, E
    VITIS, 2003, 42 (03) : 151 - 157
  • [27] A Modified Molecular Beacons-Based Multiplex Real-Time PCR Assay for Simultaneous Detection of Eight Foodborne Pathogens in a Single Reaction and Its Application
    Hu, Qinghua
    Lyu, DongYue
    Shi, Xiaolu
    Jiang, Yixiang
    Lin, Yiman
    Li, Yinghui
    Qiu, Yaqun
    He, Lianhua
    Zhang, Ran
    Li, Qingge
    FOODBORNE PATHOGENS AND DISEASE, 2014, 11 (03) : 207 - 214
  • [28] Development of an EvaGreen-based multiplex real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of six viral pathogens of porcine reproductive and respiratory disorder
    Rao, Pinbin
    Wu, Haigang
    Jiang, Yonghou
    Opriessnig, Tanja
    Zheng, Xiaowen
    Mo, Yecheng
    Yang, Zongqi
    JOURNAL OF VIROLOGICAL METHODS, 2014, 208 : 56 - 62
  • [29] MOLECULAR-CLONING AND CHARACTERIZATION OF CONTIGUOUSLY LOCATED REPETITIVE AND SINGLE-COPY DNA-SEQUENCES OF MYCOBACTERIUM-TUBERCULOSIS - DEVELOPMENT OF PCR-BASED DIAGNOSTIC ASSAY
    REDDI, PP
    TALWAR, GP
    KHANDEKAR, PS
    INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES, 1993, 61 (02) : 227 - 235
  • [30] Performance Evaluation of the new real-time PCR-based cobas HCV assay for use on the cobas 6800/8800 systems for the detection and quantification of HCV RNA
    Vermehren, Johannes
    Berkowski, Caterina
    Maasoumy, Benjamin
    Michel-Treil, Veronique
    Marino, Enrique
    Marins, Ed G.
    Wedemeyer, Heiner
    Sarrazin, Christoph
    HEPATOLOGY, 2016, 64 : 414A - 415A