Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups

被引:20
|
作者
Fraisier, Christophe [1 ]
Stor, Richard [1 ]
Tenebray, Bernard [1 ]
Sanson, Yannick [1 ]
Nicolas, Pierre [1 ]
机构
[1] IMTSSA, Unite Meningocoque, IRBA, WHO,Collaborating Ctr, F-13262 Marseille 07, France
关键词
MENINGOCOCCAL MENINGITIS; IDENTIFICATION; NIGER; CAPSULE; DISEASE;
D O I
10.1128/JCM.02415-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.
引用
收藏
页码:2662 / 2666
页数:5
相关论文
共 40 条
  • [1] Consecutive use of two multiplex PCR-based assays for simultaneous identification and determination of capsular status of nine common Neisseria meningitidis serogroups associated with invasive disease
    Bennett, DE
    Cafferkey, MT
    JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (03) : 1127 - 1131
  • [2] Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis
    Taha, MK
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) : 855 - 857
  • [3] PCR-based assny for detection of Neisseria meningitidis capsular serogroups 29E, X, and Z
    Bennett, DE
    Mulhall, RM
    Cafferkey, MT
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) : 1764 - 1765
  • [4] Simultaneous single-tube PCR-based assay for the direct identification of the five most common meningococcal serogroups from clinical samples
    Drakopoulou, Zacharoula
    Kesanopoulos, Konstantinos
    Sioumala, Maria
    Tambaki, Alexandra
    Kremastinou, Jenny
    Tzanakaki, Georgina
    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2008, 53 (02): : 178 - 182
  • [5] Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR-Based Reverse Line Blot Assay
    McKechnie, Michelle L.
    Hillman, Richard
    Couldwell, Deborah
    Kong, Fanrong
    Freedman, Eleanor
    Wang, Hui
    Gilbert, Gwendolyn L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (06) : 1871 - 1877
  • [6] Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
    de Filippis, Ivano
    de Andrade, Claudia Ferreira
    Caldeira, Nathalia
    de Azevedo, Aline Carvalho
    de Almeida, Antonio Eugenio
    BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2016, 20 (04): : 335 - 341
  • [7] Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K, Streptococcus agalactiae, and Streptococcus pneumoniae
    Hemmati, Nastaran
    Nikkhahi, Farhad
    Javadi, Amir
    Eskandarion, Sahar
    Marashi, Seyed Mahmoud Amin
    IRANIAN JOURNAL OF MICROBIOLOGY, 2021, 13 (04) : 464 - 469
  • [8] The feasibility to use minimal tumor samples for mutation analysis by multiplex PCR-based assay
    Tambo, Yuichi
    Katayama, Ryohei
    Yanagitani, Noriko
    Horiike, Atsushi
    Ohyanagi, Fumiyoshi
    Kudo, Keita
    Nishizawa, Hironari
    Uenami, Takeshi
    Gyotoku, Hiroshi
    Kobayashi, Hiroshi
    Fujita, Naoya
    Okumura, Sakae
    Ishikawa, Yuichi
    Horai, Takeshi
    Dias-Santagata, Dora
    Nishio, Makoto
    JOURNAL OF CLINICAL ONCOLOGY, 2014, 32 (15)
  • [9] Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae
    Tzanakaki, G
    Tsopanomichalou, M
    Kesanopoulos, K
    Matzourani, R
    Sioumala, M
    Tabaki, A
    Kremastinou, J
    CLINICAL MICROBIOLOGY AND INFECTION, 2005, 11 (05) : 386 - 390
  • [10] A Multiplex PCR-Based Assay for the Simultaneous Detection of Bacterial and Viral Pathogens Causing Meningitis in Children 841
    Joseph Z Glustein
    Yingze Zhang
    Garth D Ehrlich
    Michael C Gorry
    Pediatric Research, 1998, 43 (Suppl 4) : 146 - 146