Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1

被引：32

作者：

Machida, Shinichi ^{[1
]}

Takaku, Motoki ^{[1
]}

Ikura, Masae ^{[2
]}

Sun, Jiying ^{[3
]}

Suzuki, Hidekazu ^{[3
]}

Kobayashi, Wataru ^{[1
]}

Kinomura, Aiko ^{[3
]}

Osakabe, Akihisa ^{[1
]}

Tachiwana, Hiroaki ^{[1
]}

Horikoshi, Yasunori ^{[3
]}

Fukuto, Atsuhiko ^{[3
]}

Matsuda, Ryo ^{[2
]}

Ura, Kiyoe ^{[4
]}

Tashiro, Satoshi ^{[3
]}

Ikura, Tsuyoshi ^{[2
]}

Kurumizaka, Hitoshi ^{[1
]}

机构：

[1] Waseda Univ, Grad Sch Adv Sci & Engn, Struct Biol Lab, Shinjuku Ku, Tokyo 1628480, Japan

[2] Kyoto Univ, Dept Mutagenesis, Div Chromatin Regulatory Network, Radiat Biol Ctr,Sakyo Ku, Kyoto 6068501, Japan

[3] Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Cellular Biol, Minami Ku, Hiroshima 7348553, Japan

[4] Osaka Univ, Div Gene Therapy Sci, Grad Sch Med, Suita, Osaka 5650871, Japan

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

NUCLEOSOME ASSEMBLY PROTEIN-1; DNA-DAMAGE; CHAPERONES; REPAIR; BINDING; VARIANT; DIMER; DYNAMICS; YEAST; NASP;

D O I：

10.1038/srep04863

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Homologous recombination plays essential roles in mitotic DNA double strand break (DSB) repair and meiotic genetic recombination. In eukaryotes, RAD51 promotes the central homologous-pairing step during homologous recombination, but is not sufficient to overcome the reaction barrier imposed by nucleosomes. RAD54, a member of the ATP-dependent nucleosome remodeling factor family, is required to promote the RAD51-mediated homologous pairing in nucleosomal DNA. In higher eukaryotes, most nucleosomes form higher-ordered chromatin containing the linker histone H1. However, the mechanism by which RAD51/RAD54-mediated homologous pairing occurs in higher-ordered chromatin has not been elucidated. In this study, we found that a histone chaperone, Nap1, accumulates on DSB sites in human cells, and DSB repair is substantially decreased in Nap1-knockdown cells. We determined that Nap1 binds to RAD54, enhances the RAD54-mediated nucleosome remodeling by evicting histone H1, and eventually stimulates the RAD51-mediated homologous pairing in higher-ordered chromatin containing histone H1.

引用

页数：11

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