Characterization of nine microsatellite loci in Cynoglossum officinale (Boraginaceae)

被引：2
|
作者：
Korbecka, G
Wolff, K
机构：
[1] Leiden Univ, Inst Biol, NL-2300 RA Leiden, Netherlands
[2] Univ Newcastle Upon Tyne, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期
关键词：
Boraginaceae; Cynoglossum officinale; genetic structure; inbreeding; microsatellites; multiplex PCR;
D O I：
10.1111/j.1471-8286.2004.00625.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cynoglossum officinale is a biennial plant pollinated by bumblebees. We developed microsatelllite loci in order to study the population genetic structure and effects of inbreeding in this species. In this paper, we describe nine polymorphic microsatellites for C. officinale. Between two and four alleles per locus were observed in a sample of 20 individuals from one population. Multiplexing allowed the seven most useful loci to be genotyped using three polymerase chain reaction (PCR) reactions.
引用
收藏
页码：229 / 230
页数：2
相关论文
共 50 条
  • [21] Development of Nine Markers and Characterization of the Microsatellite Loci in the Endangered Gymnogobius isaza (Gobiidae)
    Araki, Kiwako S.
    Nakazawa, Takefumi
    Kawakita, Atsushi
    Kudoh, Hiroshi
    Okuda, Noboru
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (05) : 5700 - 5705
  • [22] Isolation and characterization of nine polymorphic microsatellite loci in the northern crayfish (Orconectes virilis)
    B. W. Williams
    C. S. Davis
    D. W. Coltman
    Conservation Genetics Resources, 2010, 2 : 235 - 237
  • [23] Isolation and characterization of nine polymorphic microsatellite loci in the northern crayfish (Orconectes virilis)
    Williams, B. W.
    Davis, C. S.
    Coltman, D. W.
    CONSERVATION GENETICS RESOURCES, 2010, 2 (01) : 235 - 237
  • [24] CYNOGLOSSUM-OFFICINALE TOXICITY IN CALVES
    BAKER, DC
    PFISTER, JA
    MOLYNEUX, RJ
    KECHELE, P
    JOURNAL OF COMPARATIVE PATHOLOGY, 1991, 104 (04) : 403 - 410
  • [25] Cytogenetic analysis of genotoxicity of Cynoglossum officinale L. (Boraginaceae) extract from Bosnia and Herzegovina (W. Balkan)
    Redzic, A.
    Redzic, S.
    Prazina, N.
    PLANTA MEDICA, 2011, 77 (12) : 1429 - 1429
  • [26] Isolation and characterization of nine microsatellite loci in the endangered Worthen’s Sparrow (Spizella wortheni)
    Julio Canales-Delgadillo
    Laura Scott-Morales
    Oliver Niehuis
    Judith Korb
    Conservation Genetics Resources, 2010, 2 : 151 - 153
  • [27] Isolation and characterization of nine new polymorphic microsatellite loci for blue whales (Balaenoptera musculus)
    Torres-Florez, J. P.
    Hucke-Gaete, R.
    Rosenbaum, H.
    Figueroa, C. C.
    CONSERVATION GENETICS RESOURCES, 2012, 4 (04) : 1023 - 1025
  • [28] Isolation and characterization of nine microsatellite loci in the endangered Worthen's Sparrow (Spizella wortheni)
    Canales-Delgadillo, Julio
    Scott-Morales, Laura
    Niehuis, Oliver
    Korb, Judith
    CONSERVATION GENETICS RESOURCES, 2010, 2 (01) : 151 - 153
  • [29] Isolation and characterization of nine polymorphic microsatellite loci from the desert locust, Schistocerca gregaria
    Kaatz, H. -H.
    Ferenz, H. -J.
    Langer, B.
    Moritz, R. F. A.
    MOLECULAR ECOLOGY NOTES, 2007, 7 (06): : 1042 - 1044
  • [30] Isolation and characterization of a first set of nine polymorphic microsatellite loci in Pongamia pinnata (Fabaceae)
    Sharma, Shyam Sundar
    Islam, Md Aminul
    Negi, Madan Singh
    Tripathi, Shashi Bhushan
    JOURNAL OF GENETICS, 2015, 94 : E70 - E74
12345