Characterization of nine microsatellite loci in Cynoglossum officinale (Boraginaceae)

被引：2
|
作者：
Korbecka, G
Wolff, K
机构：
[1] Leiden Univ, Inst Biol, NL-2300 RA Leiden, Netherlands
[2] Univ Newcastle Upon Tyne, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期
关键词：
Boraginaceae; Cynoglossum officinale; genetic structure; inbreeding; microsatellites; multiplex PCR;
D O I：
10.1111/j.1471-8286.2004.00625.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cynoglossum officinale is a biennial plant pollinated by bumblebees. We developed microsatelllite loci in order to study the population genetic structure and effects of inbreeding in this species. In this paper, we describe nine polymorphic microsatellites for C. officinale. Between two and four alleles per locus were observed in a sample of 20 individuals from one population. Multiplexing allowed the seven most useful loci to be genotyped using three polymerase chain reaction (PCR) reactions.
引用
收藏
页码：229 / 230
页数：2
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