C21 steroidal glycosides from Cynanchum stauntonii induce apoptosis in HepG2 cells

被引：14

作者：

Yin, Zhi-Qi ^{[1
,2
]}

Yu, Shu-Le ^{[1
,2
,3
]}

Wei, Yu-Jian ^{[4
]}

Ma, Lin ^{[1
,2
,3
]}

Wu, Zheng-Feng ^{[1
,2
,3
]}

Wang, Lei ^{[5
,6
]}

Zhang, Qing-Wen ^{[7
,8
]}

Zhao, Ming ^{[9
,10
]}

Ye, Wen-Cai ^{[5
,6
]}

Che, Chun-Tao ^{[9
,10
]}

Zhang, Jian ^{[3
]}

机构：

[1] China Pharmaceut Univ, Dept Nat Med Chem, 24 Tongjiaxiang, Nanjing 210009, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, State Key Lab Nat Med, 24 Tongjiaxiang, Nanjing 210009, Jiangsu, Peoples R China

[3] Jiangsu Prov Acad Tradit Chinese Med, Lab Translat Med, 100 Shizi St,Hongshan Rd, Nanjing 210028, Jiangsu, Peoples R China

[4] Nanjing Med Univ, Inst Clin Med 1, Nanjing 210000, Jiangsu, Peoples R China

[5] Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China

[6] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Guangdong, Peoples R China

[7] Univ Macau, Estate Key Lab Qual Res Chinese Med, Macao Sar, Peoples R China

[8] Univ Macau, Inst Chinese Med Sci, Macao Sar, Peoples R China

[9] Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA

[10] Univ Illinois, Coll Pharm, WHO Collaborating Ctr Tradit Med, Chicago, IL 60612 USA

来源：

STEROIDS | 2016年 / 106卷

基金：

中国国家自然科学基金;

关键词：

Cynanchum stauntonii; C-21 steroidal glycoside; Apoptosis; Caspase; AERIAL PART; ROOTS;

D O I：

10.1016/j.steroids.2015.12.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two new (1-2) and three known (3-5) C-21 steroidal glycosides were isolated from Cynanchum stauntonii. Their structures were elucidated on the basis of 1D and 2D-NMR spectroscopic data as well as HRTOFMS analysis. The cytotoxicity of the compounds against A549, HepG2, and 4T1 cell lines were evaluated by MIT assay. Compound 4 exhibited good inhibitory activities with the IC50 values 26.82, 12.24, and 44.12 M, respectively. Furthermore, compound 4 could induce G1 phase arrest, upregulate the expression levels of caspases-3, -9, and Bax, and downregulate the expression level of Bc1-2. These results indicated that compound 4 might be valuable to anticancer drug candidates. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：55 / 61

页数：7

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