Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

被引：8
|
作者：
Pardini, Manuela
Giannoni, Federico
Palma, Carla
Iona, Elisabetta
Cafaro, Aurelio
Brunori, Lara
Rinaldi, Monica
Fazio, Vito Michele
Laguardia, Maria Elena
Carbonella, Damiano Cosimo
Magnani, Mauro
Ensoli, Barbara
Fattorini, Lanfranco
Cassone, Antonio
机构：
[1] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy
[2] Ist Super Sanita, Ctr Nazl AIDS, I-00161 Rome, Italy
[3] CNR, Area Tor Vergata, Ist Neurol & Med Mol, Rome, Italy
[4] CIR, Sez Med Mol & Biotecnol, Rome, Italy
[5] Univ Urbino, Ist Chim Biol G Fornaini, I-61029 Urbino, Italy
来源：
FEMS MICROBIOLOGY LETTERS | 2006年 / 262卷 / 02期
关键词：
tuberculosis; DNA vaccines; 85B antigen; HIV-Tat; mice protection; immune responses;
D O I：
10.1111/j.1574-6968.2006.00390.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung(-1) in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guerin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
引用
收藏
页码：210 / 215
页数：6
相关论文
共 50 条
  • [31] Phagosomal processing of Mycobacterium tuberculosis antigen 85B is modulated independently of mycobacterial viability and phagosome maturation (vol 73, pg 1097, 2005)
    Ramachandra, L
    Smialek, JL
    Shank, SS
    Convery, M
    Boom, WH
    Harding, CV
    INFECTION AND IMMUNITY, 2006, 74 (01) : 802 - 802
  • [32] Reliable naked-eye detection of Mycobacterium tuberculosis antigen 85B using gold and copper nanoshell-enhanced immunoblotting techniques
    Le Minh Tu Phan
    Kim, Eun Bee
    Cheon, Seon Ah
    Shim, Tae Sun
    Kim, Hwa-Jung
    Park, Tae Jung
    SENSORS AND ACTUATORS B-CHEMICAL, 2020, 317
  • [33] Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis
    Kamath, AT
    Feng, CG
    Macdonald, M
    Briscoe, H
    Britton, WJ
    INFECTION AND IMMUNITY, 1999, 67 (04) : 1702 - 1707
  • [34] Enhancement of immune response to a DNA vaccine against Mycobacterium tuberculosis Ag85B by incorporation of an autophagy inducing system
    Meerak, Jomkhwan
    Wanichwecharungruang, Supason P.
    Palaga, Tanapat
    VACCINE, 2013, 31 (05) : 784 - 790
  • [35] Protective efficacy of a recombinant BCG secreting antigen 85B/Rv3425 fusion protein against Mycobacterium tuberculosis infection in mice
    Wang, Jiuling
    Qie, Yaqing
    Liu, Wei
    Wang, Honghai
    HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2012, 8 (12) : 1869 - 1874
  • [36] A Comparative Approach to Strategies for Cloning, Expression, and Purification of Mycobacterium tuberculosis Mycolyl Transferase 85B and Evaluation of Immune Responses in BALB/c Mice
    Aghababa, Haniyeh
    Mobarez, Ashraf Mohabati
    Khoramabadi, Nima
    Behmanesh, Mehrdad
    Mahdavi, Mehdi
    Tebianian, Majid
    Nejati, Mehdi
    MOLECULAR BIOTECHNOLOGY, 2014, 56 (06) : 487 - 497
  • [37] A Comparative Approach to Strategies for Cloning, Expression, and Purification of Mycobacterium tuberculosis Mycolyl Transferase 85B and Evaluation of Immune Responses in BALB/c Mice
    Haniyeh Aghababa
    Ashraf Mohabati Mobarez
    Nima Khoramabadi
    Mehrdad Behmanesh
    Mehdi Mahdavi
    Majid Tebianian
    Mehdi Nejati
    Molecular Biotechnology, 2014, 56 : 487 - 497
  • [38] HLA-DR binding prediction and experimental evaluation of T-cell epitopes of mycolyl transferase 85B (Ag85B), a major secreted antigen of Mycobacterium tuberculosis
    Mustafa, AS
    Abal, AT
    Shaban, F
    El-Shamy, AM
    Amoudy, HA
    MEDICAL PRINCIPLES AND PRACTICE, 2005, 14 (03) : 140 - 146
  • [39] Diagnosis of pulmonary and extrapulmonary tuberculosis based on detection of mycobacterial antigen 85B by immuno-PCR
    Singh, Netrapal
    Sreenivas, Vishnubhatla
    Gupta, Krishna B.
    Chaudhary, Anil
    Mittal, Anshu
    Varma-Basil, Mandira
    Prasad, Rajendra
    Gakhar, Surender K.
    Khuller, Gopal K.
    Mehta, Promod K.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2015, 83 (04) : 359 - 364
  • [40] Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice
    Li Sun
    Quan Yuan
    Tianhua Xu
    Li Yao
    Jiangmin Feng
    Jianfei Ma
    Lining Wang
    Changlong Lv
    Danan Wang
    Biotechnology Letters, 2017, 39 : 1159 - 1166
12345