Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

被引：8
|
作者：
Pardini, Manuela
Giannoni, Federico
Palma, Carla
Iona, Elisabetta
Cafaro, Aurelio
Brunori, Lara
Rinaldi, Monica
Fazio, Vito Michele
Laguardia, Maria Elena
Carbonella, Damiano Cosimo
Magnani, Mauro
Ensoli, Barbara
Fattorini, Lanfranco
Cassone, Antonio
机构：
[1] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy
[2] Ist Super Sanita, Ctr Nazl AIDS, I-00161 Rome, Italy
[3] CNR, Area Tor Vergata, Ist Neurol & Med Mol, Rome, Italy
[4] CIR, Sez Med Mol & Biotecnol, Rome, Italy
[5] Univ Urbino, Ist Chim Biol G Fornaini, I-61029 Urbino, Italy
来源：
FEMS MICROBIOLOGY LETTERS | 2006年 / 262卷 / 02期
关键词：
tuberculosis; DNA vaccines; 85B antigen; HIV-Tat; mice protection; immune responses;
D O I：
10.1111/j.1574-6968.2006.00390.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung(-1) in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guerin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
引用
收藏
页码：210 / 215
页数：6
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