Rapid detection of Brucella species in cattle semen by PCR

被引：7

作者：

Kaushik, P. ^{[1
]}

Singh, D. K. ^{[1
]}

Tiwari, A. K. ^{[1
]}

Kataria, R. S. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Vet Publ Hlth, Izatnagar 243122, Uttar Pradesh, India

来源：

JOURNAL OF APPLIED ANIMAL RESEARCH | 2006年 / 30卷 / 01期

关键词：

Brucella; cattle; PCR; semen;

D O I：

10.1080/09712119.2006.9706818

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

To overcome the problems of sensitivity and specificity associated with serological tests and difficulty in isolation of bacteria for diagnosing brucellosis, a PCR assay was standardized using specific primer from the gene encoding 31 kDa protein for Brucella species with genomic DNA isolated from the overnight grown cultures of B. abortus S99. The PCR could detect the, Brucella organism up to 40 cfu per ml in cattle semen. Reprecipitation of DNA with butanol increased sensitivity of PCR.

引用

页码：25 / 28

页数：4

共 50 条

[41] Evaluation of a PCR test for the diagnosis of Brucella ovis infection in semen samples from rams
Manterola, L
Tejero-Garcés, A
Ficapal, A
Shopayeva, G
Blasco, JM
Marin, CM
López-Goñi, I
VETERINARY MICROBIOLOGY, 2003, 92 (1-2) : 65 - 72
[42] Characterization and evaluation of an arbitrary primed Polymerase Chain Reaction (PCR) product for the specific detection of Brucella species
Qasem, Jafar A.
AlMomin, Sabah
Al-Mouqati, Salwa A.
Kumar, Vinod
SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2015, 22 (02) : 220 - 226
[43] Detection of Nontuberculous Mycobacterial Species from Tissue Samples of Cattle and Buffaloes by PCR and PRA (PCR-RFLP)
Slathia, Pallvi
Narang, Deepti
Chandra, Mudit
INDIAN JOURNAL OF ANIMAL RESEARCH, 2022, 56 (06) : 730 - 735
[44] Detection of Brucella melitensis in semen using the polymerase chain reaction assay
Amin, AS
Hamdy, MER
Ibrahim, AK
VETERINARY MICROBIOLOGY, 2001, 83 (01) : 37 - 44
[45] Molecular typing of Brucella species by PCR-RFLP and SSCP
Hemade, M. V.
Gandge, R. S.
INDIAN JOURNAL OF ANIMAL SCIENCES, 2016, 86 (05): : 502 - 507
[46] Detection of Coxiella burnetii in cattle by PCR
Kirkan, Suekrue
Kaya, Osman
Tekbiyik, Serten
Parin, Ugur
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2008, 32 (03): : 215 - 220
[47] Differential Detection of Hemotropic Mycoplasma Species in Cattle by Melting Curve Analysis of PCR Products
Nishizawa, Ikuo
Sato, Makoto
Fujihara, Masatoshi
Sato, Shigeru
Harasawa, Ryo
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2010, 72 (01): : 77 - 79
[48] A NOVEL MULTIPLEX PCR ASSAY FOR RAPID DETECTION OF CLINICALLY RELEVANT ASPERGILLUS SPECIES
Li, S.
Wang, Z.
Zhang, Y.
Wang, Y.
Wang, H.
Su, Y.
Zhou, Z.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2022, 31 : S40 - S41
[49] Rapid detection and identification of Stachybotrys and Chaetomium species using tissue PCR analysis
Lewinska, Anna M.
Peuhkuri, Ruut H.
Rode, Carsten
Andersen, Birgitte
Hoof, Jakob B.
JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 130 : 115 - 122
[50] Rapid differentiation and in situ detection of 16 sourdough Lactobacillus species by multiplex PCR
Settanni, L
van Sinderen, D
Rossi, J
Corsetti, A
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (06) : 3049 - 3059

← 1 2 3 4 5 →