Development of a Simultaneous Detection Method for Foodborne Pathogens Using Surface Plasmon Resonance Biosensors

被引：10

作者：

Zhang, Xiaoguang ^{[1
]}

Kitaoka, Hayato ^{[1
]}

Tsuni, Sachiko ^{[1
]}

Tamai, Mitsuru ^{[1
]}

Kobayashi, Hiroshi ^{[2
]}

Honjoh, Ken-ichi ^{[3
]}

Miyamoto, Takahisa ^{[3
]}

机构：

[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Dept Biosci & Biotechnol, Higashi Ku, Fukuoka 8128581, Japan

[2] Fukuoka Womens Univ, Int Coll Arts & Sci, Dept Food & Hlth Sci, Higashi Ku, Fukuoka 8138529, Japan

[3] Kyushu Univ, Fac Agr, Grad Sch, Dept Biosci & Biotechnol, Fukuoka 812, Japan

来源：

FOOD SCIENCE AND TECHNOLOGY RESEARCH | 2014年 / 20卷 / 02期

关键词：

simultaneous detection; foodborne pathogens; SPR; Escherichia colt O157:H7; Salmonella; Listeria; LINKED-IMMUNOSORBENT-ASSAY; ESCHERICHIA-COLI O157-H7; LISTERIA-MONOCYTOGENES; SALMONELLA; IDENTIFICATION; IMMUNOSENSOR; AUREUS;

D O I：

10.3136/fstr.20.317

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (0157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 x 10(6), 1.8 x 10(6), and 0.7 x 10(7) CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 10(5) to 10(8) CFU/mL.

引用

页码：317 / 325

页数：9

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