Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

被引：17
|
作者：
Sekar, Raju [1 ]
Kaczmarsky, Longin T. [1 ]
Richardson, Laurie L. [1 ]
机构：
[1] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 08期
关键词：
GRADIENT GEL-ELECTROPHORESIS; COMMUNITY COMPOSITION; SIDERASTREA-SIDEREA; FILAMENTOUS-SULFUR; CYANOBACTERIA; RED;
D O I：
10.1128/AEM.01500-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.
引用
收藏
页码：2581 / 2584
页数：4
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