Isolation and characterization of chitinase from a marine bacterium Vibrio sp.

被引：10
|
作者：
Zhou, SN [1 ]
Yang, CY
Lu, YJ
Huang, L
Cai, CH
Lin, YC
机构：
[1] Zhongshan Univ, Dept Biochem, State Key Lab Biocontrol, Guangzhou, Peoples R China
[2] Acad Sinica, Inst Oceanol, S China Sea, Beijing, Peoples R China
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1999年 / 15卷 / 06期
基金：
中国国家自然科学基金;
关键词：
chitinase; marine bacteria; Vibrio sp;
D O I：
10.1023/A:1008932217926
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A chitinase was separated from the culture broth of Vibrio sp. 11211 isolated from sediment from the South China Sea. The chitinase was purified 18.3-fold with 33% recovery by ammonium sulphate precipitation and chromatography. The subunit molecular weight of the enzyme was estimated by SDS-PAGE to be about 30 kDa. The enzyme showed optimum pH at 6.5 and optimum temperature at 50 degrees C, and was stable in the pH range of 4 to 9 and at the temperature below 40 degrees C.
引用
收藏
页码：745 / 746
页数：2
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