Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation

被引：24
|
作者：
Koh, Hyun Seok [1 ]
Kim, Gyoung Hee [2 ]
Lee, Young Sun [1 ]
Koh, Young Jin [2 ]
Jung, Jae Sung [1 ]
机构：
[1] Sunchon Natl Univ, Dept Biol, Sunchon 540950, South Korea
[2] Sunchon Natl Univ, Dept Plant Med, Sunchon 540950, South Korea
来源：
PLANT PATHOLOGY JOURNAL | 2014年 / 30卷 / 01期
关键词：
bacterial canker; kiwifruit; multiplex PCR; PCR detection; Pseudomonas syringae pv. actinidiae; BACTERIAL CANKER; 1ST REPORT; CAUSAL AGENT; KIWIFRUIT; GENE; POPULATIONS; RELATEDNESS; CHINENSIS; PATHOVARS; SEQUENCE;
D O I：
10.5423/PPJ.NT.09.2013.0095
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The molecular features of Pseudomonas syringae pv. actinidiae strains isolated in Korea were compared with strains isolated in Japan and Italy. Sequencing of eight P. syringae pv. actinidiae and three P. syringae pv. theae strains revealed a total of 44 single nucleotide polymorphisms across 4,818 bp of the concatenated alignment of nine genes. A multiplex PCR assay was developed for the detection of P. syringae pv. actinidiae and for the specific detection of recent haplotype strains other than strains isolated since the 1980s in Korea. The primer pair, designated as TacF and TacR, specifically amplified a 545-bp fragment with the genomic DNA of new haplotype of P. syringae pv. actinidiae strains. A multiplex PCR conducted with the TacF/TacR primer pair and the universal primer pair for all P. syringae pv. actinidiae strains can be simultaneously applied for the detection of P. syringae pv. actinidiae and for the differentiation of new haplotype strains.
引用
收藏
页码：96 / 101
页数：6
相关论文
共 50 条
  • [41] Outbreak of bacterial canker on Hort16A (Actinidia chinensis Planchon) caused by Pseudomonas syringae pv. actinidiae in Korea
    Koh, Y. J.
    Kim, G. H.
    Jung, J. S.
    Lee, Y. S.
    Hur, J. S.
    NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE, 2010, 38 (04) : 275 - 282
  • [42] DEVELOPMENT OF A MULTIPLEX PCR ASSAY FOR THE SIMULTANEOUS DETECTION OF CLAVIBACTER MICHIGANENSIS subsp MICHIGANENSIS, PSEUDOMONAS SYRINGAE pv. TOMATO AND XANTHOMONAS AXONOPODIS pv. VESICATORIA USING PURE CULTURES
    Ozdemir, Z.
    JOURNAL OF PLANT PATHOLOGY, 2009, 91 (02) : 495 - 497
  • [43] Aspects about virulence and epiphytic fitness of Pseudomonas syringae pv. syringae strains isolated from mango trees
    Arrebola, E.
    Carrion, V.
    Codina, J. C.
    Perez-Garcia, A.
    Cazorla, F. M.
    de Vicente, A.
    MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS, 2011, : 16 - 20
  • [44] CHARACTERIZATION OF PSEUDOMONAS SYRINGAE pv. SYRINGAE ISOLATED FROM MANGO IN SICILY AND OCCURRENCE OF COPPER-RESISTANT STRAINS
    Aiello, D.
    Ferrante, P.
    Vitale, A.
    Polizzi, G.
    Scortichini, M.
    Cirvilleri, G.
    JOURNAL OF PLANT PATHOLOGY, 2015, 97 (02) : 273 - 282
  • [45] Short communication.: Detection by multiplex PCR and characterization of nontoxigenic strains of Pseudomonas syringae pv. phaseolicola from different places in Spain
    Rico, A.
    Erdozain, M.
    Ortiz-Barredo, A.
    Ruiz de Galarreta, J. I.
    Murillo, J.
    SPANISH JOURNAL OF AGRICULTURAL RESEARCH, 2006, 4 (03) : 261 - 267
  • [46] Unraveling the Molecular Interaction between Pseudomonas syringae pv. actinidiae (Psa) and the Kiwifruit Plant through RNAseq Approach
    Michelotti, V.
    Lamontanara, A.
    Buriani, G.
    Cellini, A.
    Donati, I.
    Vanneste, J. L.
    Cattivelli, L.
    Spinelli, F.
    Orru, L. I.
    Tacconi, G.
    I INTERNATIONAL SYMPOSIUM ON BACTERIAL CANKER OF KIWIFRUIT, 2015, 1095 : 89 - 94
  • [47] Molecular Characterization and Taxonomic Assignment of Three Phage Isolates from a Collection Infecting Pseudomonas syringae pv. actinidiae and P. syringae pv. phaseolicola from Northern Italy
    Martino, Gabriele
    Holtappels, Dominique
    Vallino, Marta
    Chiapello, Marco
    Turina, Massimo
    Lavigne, Rob
    Wagemans, Jeroen
    Ciuffo, Marina
    VIRUSES-BASEL, 2021, 13 (10):
  • [48] Draft Genome Resources Sequences of Six Pseudomonas syringae pv. actinidiae Strains Isolated from Actinidia chinensis var. deliciosa Leaves in Portugal
    Ares, Aitana
    Tacao, Marta
    Figueira, Daniela
    Garcia, Eva
    Costa, Joana
    PHYTOPATHOLOGY, 2021, 111 (01) : 237 - 239
  • [49] Comparative genomic analyses provide insight into the pathogenicity of three Pseudomonas syringae pv. actinidiae strains from Anhui Province, China
    Wang, Qian
    Zhang, Yiju
    Chen, Rui
    Zhang, Lei
    Fu, Min
    Zhang, Lixin
    BMC GENOMICS, 2024, 25 (01):
  • [50] Genomic and pathogenic properties of Pseudomonas syringae pv. syringae strains isolated from apricot in East Azerbaijan province, Iran
    Vasebi, Yalda
    Khakvar, Reza
    Faghihi, Mohammad Mehdi
    Vinatzer, Boris A.
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2019, 19
12345