Inhibition of HCV replication in HCV replicon by shRNAs

被引：3

作者：

Hamazaki, Hiroyuki

Takahashi, Hitoshi

Shimotohno, Kunitada

Miyano-Kurosaki, Naoko

Takaku, Hiroshi

机构：

[1] Chiba Inst Technol, Dept Life & Environm Sci, Narashino, Chiba 2750016, Japan

[2] Chiba Inst Technol, High Technol Res Ctr, Narashino, Chiba 2750016, Japan

[3] Kyoto Univ, Inst Virus Res, Dept Viral Oncol, Kyoto 606, Japan

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2006年 / 25卷 / 07期

关键词：

RNAi; long dsRNA; shRNA; HCVIRES/core; HCV replicon; anti-HCV;

D O I：

10.1080/15257770600726091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that the vector-derived long dsRNA specifically inhibits the replication of HCV RNA in HCV replicon. We designed a long dsRNA targeted to the full-length HCV IRES/core elements (1-to 377-nt). Our results revealed that the replication of HCV RNA was reduced to near background levels in a sequence-specific manner by the long dsRNAs in the HCV replicon. We also designed four shRNAs against several regions (120- to 139-nt, 260- to 279-nt, 330- to 349-nt, and 340- to 359-nt) of the HCV IRES/Core elements. The two HCV IRES/core-specific shRNAs, 330- to 349-nt and 340- to 359-nt, containing the AUG initiation codon sequence showed stronger HCV inhibitory effects than the other two shRNAs, 120- to 139-nt and 260- to 279-nt.

引用

页码：801 / 805

页数：5

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