Inhibition of HCV replication in HCV replicon by shRNAs

被引：3
|
作者：
Hamazaki, Hiroyuki
Takahashi, Hitoshi
Shimotohno, Kunitada
Miyano-Kurosaki, Naoko
Takaku, Hiroshi
机构：
[1] Chiba Inst Technol, Dept Life & Environm Sci, Narashino, Chiba 2750016, Japan
[2] Chiba Inst Technol, High Technol Res Ctr, Narashino, Chiba 2750016, Japan
[3] Kyoto Univ, Inst Virus Res, Dept Viral Oncol, Kyoto 606, Japan
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2006年 / 25卷 / 07期
关键词：
RNAi; long dsRNA; shRNA; HCVIRES/core; HCV replicon; anti-HCV;
D O I：
10.1080/15257770600726091
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We show that the vector-derived long dsRNA specifically inhibits the replication of HCV RNA in HCV replicon. We designed a long dsRNA targeted to the full-length HCV IRES/core elements (1-to 377-nt). Our results revealed that the replication of HCV RNA was reduced to near background levels in a sequence-specific manner by the long dsRNAs in the HCV replicon. We also designed four shRNAs against several regions (120- to 139-nt, 260- to 279-nt, 330- to 349-nt, and 340- to 359-nt) of the HCV IRES/Core elements. The two HCV IRES/core-specific shRNAs, 330- to 349-nt and 340- to 359-nt, containing the AUG initiation codon sequence showed stronger HCV inhibitory effects than the other two shRNAs, 120- to 139-nt and 260- to 279-nt.
引用
收藏
页码：801 / 805
页数：5
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