A new fluorescent pH probe for imaging lysosomes in living cells

被引：57

作者：

Lv, Hong-Shui ^{[1
]}

Huang, Shu-Ya ^{[2
]}

Xu, Yu ^{[1
]}

Dai, Xi ^{[1
]}

Miao, Jun-Ying ^{[2
]}

Zhao, Bao-Xiang ^{[1
]}

机构：

[1] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R China

[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2014年 / 24卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Rhodamine B; Fluorescent probe; pH chemodosimeter; Lysosomes imaging; RHODAMINE-B; DESIGN;

D O I：

10.1016/j.bmcl.2013.12.025

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A new rhodamine B-based pH fluorescent probe has been synthesized and characterized. The probe responds to acidic pH with short response time, high selectivity and sensitivity, and exhibits a more than 20-fold increase in fluorescence intensity within the pH range of 7.5-4.1 with the pK(a) value of 5.72, which is valuable to study acidic organelles in living cells. Also, it has been successfully applied to HeLa cells, for its low cytotoxicity, brilliant photostability, good membrane permeability and no 'alkalizing effect' on lysosomes. The results demonstrate that this probe is a lysosome-specific probe, which can selectively stain lysosomes and monitor lysosomal pH changes in living cells. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：535 / 538

页数：4

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