A new fluorescent pH probe for imaging lysosomes in living cells

被引：57
|
作者：
Lv, Hong-Shui [1 ]
Huang, Shu-Ya [2 ]
Xu, Yu [1 ]
Dai, Xi [1 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
机构：
[1] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R China
[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2014年 / 24卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Rhodamine B; Fluorescent probe; pH chemodosimeter; Lysosomes imaging; RHODAMINE-B; DESIGN;
D O I：
10.1016/j.bmcl.2013.12.025
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A new rhodamine B-based pH fluorescent probe has been synthesized and characterized. The probe responds to acidic pH with short response time, high selectivity and sensitivity, and exhibits a more than 20-fold increase in fluorescence intensity within the pH range of 7.5-4.1 with the pK(a) value of 5.72, which is valuable to study acidic organelles in living cells. Also, it has been successfully applied to HeLa cells, for its low cytotoxicity, brilliant photostability, good membrane permeability and no 'alkalizing effect' on lysosomes. The results demonstrate that this probe is a lysosome-specific probe, which can selectively stain lysosomes and monitor lysosomal pH changes in living cells. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：535 / 538
页数：4
相关论文
共 50 条
  • [1] A NBD-based simple but effective fluorescent pH probe for imaging of lysosomes in living cells
    Cao, Xiang-Jian
    Chen, Li-Na
    Zhang, Xuan
    Liu, Jin-Ting
    Chen, Ming-Yu
    Wu, Qiu-Rong
    Miao, Jun-Ying
    Zhao, Bao-Xiang
    ANALYTICA CHIMICA ACTA, 2016, 920 : 86 - 93
  • [2] A new ratiometric two-photon fluorescent probe for imaging of lysosomes in living cells and tissues
    Zhou, Liyi
    Liu, Yongchao
    Hu, Shunqin
    Wang, Haifei
    Sun, Hongyan
    Zhang, Xiaobing
    TETRAHEDRON, 2016, 72 (31) : 4637 - 4642
  • [3] A simple pH fluorescent probe based on new fluorophore indolizine for imaging of living cells
    Ge, Yanqing
    Liu, Aikun
    Dong, Jian
    Duan, Guiyun
    Cao, Xiaoqun
    Li, Fuyou
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 247 : 46 - 52
  • [4] Lysosomes-targeting near-infrared fluorescent probe for the detection of pH in living cells
    Liu, Chang
    Yan, Huimin
    Wu, Jianhong
    Wang, Zhiming
    He, Song
    Zhao, Liancheng
    Zeng, Xianshun
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 278
  • [5] A novel pH fluorescent probe based on indocyanine for imaging of living cells
    Niu, Weifen
    Nan, Ming
    Fan, Li
    Wong, Man Shing
    Shuang, Shaomin
    Dong, Chuan
    DYES AND PIGMENTS, 2016, 126 : 224 - 231
  • [6] Ratiometric Emission Fluorescent pH Probe for Imaging of Living Cells in Extreme Acidity
    Niu, Weifen
    Fan, Li
    Nan, Ming
    Li, Zengbo
    Lu, Dongtao
    Wong, Man Shing
    Shuang, Shaomin
    Dong, Chuan
    ANALYTICAL CHEMISTRY, 2015, 87 (05) : 2788 - 2793
  • [7] Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes
    Kim, Young Ju
    Jang, Mina
    Roh, Jongtae
    Lee, Yoon Jeong
    Moon, Hee Jung
    Byun, Jimin
    Wi, Jihyun
    Ko, Sung-Kyun
    Tae, Jinsung
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (20)
  • [8] A fluorescent pH probe for acidic organelles in living cells
    Chen, Jyun-Wei
    Chen, Chih-Ming
    Chang, Cheng-Chung
    ORGANIC & BIOMOLECULAR CHEMISTRY, 2017, 15 (37) : 7936 - 7943
  • [9] A convenient fluorescent probe for monitoring lysosomal pH change and imaging mitophagy in living cells
    Xu, Hongkang
    Bu, Yingcui
    Wang, JunJun
    Qu, Min
    Zhang, Jie
    Zhu, Xiaojiao
    Liu, Gang
    Wu, Zhichao
    Chen, Guangmei
    Zhou, Hongping
    SENSORS AND ACTUATORS B-CHEMICAL, 2021, 330 (330):
  • [10] A benzooxazine-based ratiometric fluorescent probe for pH imaging in living cells and bacteria
    Peng, Junxiang
    Chen, Hongxia
    Sun, Mingtai
    Yu, Huan
    Hou, Jing
    Wang, Suhua
    SENSORS AND ACTUATORS B-CHEMICAL, 2021, 335
12345