Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33

被引：5

作者：

Senchenkova, Sof'ya N. ^{[1
]}

Hou, Wenqi ^{[2
]}

Naumenko, Olesya I. ^{[1
,4
]}

Geng, Peng ^{[3
]}

Shashkov, Alexander S. ^{[1
]}

Perepelov, Andrei V. ^{[1
]}

Yang, Bin ^{[2
]}

Knirel, Yuriy A. ^{[1
]}

机构：

[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, Key Lab Mol Microbiol & Technol, Minist Educ, TEDA Inst Biol Sci & Biotechnol,TEDA, Tianjin, Peoples R China

[3] Tianjin Med Univ, Sch Basic Med Sci, Tianjin 300070, Peoples R China

[4] DI Mendeleev Univ Chem Technol Russia, Russian Acad Sci, Higher Chem Coll, Moscow, Russia

来源：

CARBOHYDRATE RESEARCH | 2018年 / 460卷

基金：

俄罗斯科学基金会;

关键词：

Escherichia coli; Lipopolysaccharide; O-antigen; O-specific polysaccharide; Bacterial polysaccharide structure; O-antigen gene cluster; Glycerol phosphate; ANTIGEN; CLUSTER; BIOSYNTHESIS;

D O I：

10.1016/j.carres.2018.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D H-1 and C-13 NMR spectroscopy: [GRAPHICS] The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：47 / 50

页数：4

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