Formation of DNA double-strand breaks and DNA-protein crosslinks by irradiation of DNA in the presence of a protein

被引：11

作者：

Distel, L ^{[1
]}

Distel, B

Schüssler, H

机构：

[1] Univ Erlangen Nurnberg, Klin Strahlentherapie, D-8520 Erlangen, Germany

[2] Univ Erlangen Nurnberg, Inst Med Phys, D-8520 Erlangen, Germany

来源：

RADIATION PHYSICS AND CHEMISTRY | 2002年 / 65卷 / 02期

关键词：

radiolysis of DNA; DNA double-strand breaks; DNA-protein crosslinks; protein radicals; constant field gel electrophoresis; nitrocellulose filter assay;

D O I：

10.1016/S0969-806X(02)00215-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Double-stranded DNA and serum albumin were irradiated with X-rays in different phosphate solutions under N-2, N2O and air. Double-strand breaks (dsb) were determined by electrophoresis. A new stochastic model makes now possible to calculate the values of DNA-protein crosslinks (Dpc) with improved accuracy from the nitrocellulose filter assay. The yields of Dpc are in the same order of magnitude as the yields of dsb. The conclusion is that secondary protein radicals and protein peroxyl radicals cause DNA dsb and Dpc. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：141 / 149

页数：9

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