Nerve Agents Assay Using Cholinesterase Based Biosensor

被引：19
|
作者：
Pohanka, Miroslav [1 ]
Dobes, Petr [3 ,4 ]
Drtinova, Lucie
Kuca, Kamil [1 ,2 ,3 ,4 ]
机构：
[1] Univ Def, Fac Mil Hlth Sci, Ctr Adv Studies, Hradec Kralove, Czech Republic
[2] Univ Def, Fac Mil Hlth Sci, Dept Toxicol, Hradec Kralove, Czech Republic
[3] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-11142 Prague, Czech Republic
[4] Univ JE Purkyne, Fac Sci, Dept Chem, Usti Nad Labem, Czech Republic
来源：
ELECTROANALYSIS | 2009年 / 21卷 / 10期
关键词：
Organophophate; Biosensor; Acetylcholinesterase; Sarin; Soman; VX; Tabun; Cyclosarin; SCREEN-PRINTED ELECTRODE; AMPEROMETRIC BIOSENSOR; PORTABLE BIOSENSOR; ACETYLCHOLINESTERASE; PERFORMANCE; PESTICIDES; SENSOR; WATER; IMMOBILIZATION; NANOPARTICLES;
D O I：
10.1002/elan.200804528
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Electrochemical biosensor based on electric eel acetylcholinesterase (ACME) (EC 3.1.1.7) was performed for assay of nerve agents - tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by it strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created front acetylthiocholine (ATChCl) by AChE-catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88 x 10(-10) M) and VX (8.51 x 10(-10) M) after one-minute assay. The biosensor was found long term stable at low as well its laboratory temperature.
引用
收藏
页码：1177 / 1182
页数：6
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