Analytical scale ultrasonic standing wave manipulation of cells and microparticles

被引：80
|
作者：
Coakley, WT [1 ]
Hawkes, JJ [1 ]
Sobanski, MA [1 ]
Cousins, CM [1 ]
Spengler, J [1 ]
机构：
[1] Cardiff Univ, Sch Biosci, Cardiff CF10 3TL, S Glam, Wales
来源：
ULTRASONICS | 2000年 / 38卷 / 1-8期
基金：
英国生物技术与生命科学研究理事会;
关键词：
acoustic streaming; antigen detection; blood separation; immunoassay; radiation pressure; ultrasonic standing wave;
D O I：
10.1016/S0041-624X(99)00151-1
中图分类号：
O42 [声学];
学科分类号：
070206 ; 082403 ;
摘要：
The ultrasonic standing-wave manipulation of suspended eukaryotic cells, bacteria and submicron latex or silica particles has been examined here. The different systems, involving plane or tubular ultrasonic transducers and a range of acoustic pathlengths, have been designed to treat suspension volumes of analytical scale i.e. 5 ml to 50 mu l for both sample batch and 'on-line' situations. Frequencies range from 1 to 12 MHz. The influence of secondary cell-cell interaction forces in determining the cell concentration dependence of harvesting efficiency in batch sedimentation systems is considered. Applications of standing wave radiation forces to (1) clarify cell suspensions, (2) enhance particle agglutination immunoassay detection of cells or cellular products and (3) examine and enhance cell-cell interactions in suspension are described. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：638 / 641
页数：4
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