Diffraction-based assay for detecting multiple analytes

被引：51
|
作者：
Goh, JB [1 ]
Loo, RW [1 ]
McAloney, RA [1 ]
Goh, MC [1 ]
机构：
[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 374卷 / 01期
关键词：
biosensor; diffraction; immunoassay; micro-contact printing; multi-analyte;
D O I：
10.1007/s00216-002-1478-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The principles of diffraction are utilized to enable the simultaneous detection of multiple analytes in solution, forming the basis of a multi-analyte sensor. Probe molecules are immobilized on a substrate such that each type of molecule defines a specific pattern within the same region of substrate. The binding of a target molecule to its complementary probe is heralded by a characteristic diffraction image. This principle is demonstrated using antibody conjugates.
引用
收藏
页码：54 / 56
页数：3
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