Diffraction-based assay for detecting multiple analytes

被引：51

作者：

Goh, JB ^{[1
]}

Loo, RW ^{[1
]}

McAloney, RA ^{[1
]}

Goh, MC ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 374卷 / 01期

关键词：

biosensor; diffraction; immunoassay; micro-contact printing; multi-analyte;

D O I：

10.1007/s00216-002-1478-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The principles of diffraction are utilized to enable the simultaneous detection of multiple analytes in solution, forming the basis of a multi-analyte sensor. Probe molecules are immobilized on a substrate such that each type of molecule defines a specific pattern within the same region of substrate. The binding of a target molecule to its complementary probe is heralded by a characteristic diffraction image. This principle is demonstrated using antibody conjugates.

引用

页码：54 / 56

页数：3

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