Effects of anaerobic granular sludge towards the treatment of flowback water in an up-flow anaerobic sludge blanket bioreactor: Comparison between mesophilic and thermophilic conditions

Cost-effective treatment of flowback water remains a challenge for the sustainability of shale gas development. This study evaluated the efficiency of anaerobic granular sludge (AnGS) technology for flowback water treatment under mesophilic and thermophilic conditions. The granule characteristics and metagenomic characterization were also investigated. Thermophilic AnGS achieved 70.9% of COD removal and 362 NmL/d of methane production, higher than those for mesophilic AnGS (60.0% and 241 NmL/d). Thermophilic AnGS had higher extracellular polymeric substances content but low granular size and settleability. Metagenomic analysis revealed the genes related to hydrolysis acidification and carbohydrate metabolism were upregulated during thermophilic condition. Thermophilic condition most likely improved the hydrolysis of complex organics in the flowback water such as guar gum and hydrolyzed polyacrylamide, and led to higher COD removal and methane production. These results suggest that AnGS technology is a promising alternative for the treatment of flowback water, particularly when operated at thermophilic condition.