Study on the interaction between gatifloxacin mesylate and salmon sperm DNA by fluorescence method

被引：11
|
作者：
Wei, Wei [1 ]
Gao, Shou-Guo [1 ]
Shi, Nan-Ning [1 ]
机构：
[1] Southeast Univ, Sch Publ Hlth, Nanjing 210019, Peoples R China
来源：
JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 2006年 / 53卷 / 03期
关键词：
salmon sperm DNA; gatifloxacin mesylate; interaction; fluorescence quenching;
D O I：
10.1002/jccs.200600095
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The interaction between gatifloxacin mesylate (GM) and salmon sperm DNA was studied by fluorescence spectrometry and ultra-violet (UV) spectrometry. Additions of salmon sperm DNA to GM solution resulted in its strong fluorescence quenching and UV absorbance decrease due to the strong interaction between GM and salmon sperm DNA. Both the maximum fluorescence emission wavelength and the maximum UV absorbance wavelength of GM did not change with the increasing concentration of salmon sperm DNA, indicating no intercalative binding existed between them. The Stem-Volmer plot indicated that the fluorescence-quenching constant at different temperatures or-different salmon sperm DNA concentration ranges was different. Effects of ionic strength and I- on the fluorescence quenching of GM by salmon sperm DNA indicated that electrostatic interaction and groove binding coexisted between them.
引用
收藏
页码：721 / 726
页数：6
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